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HIPP-T006 serum-free medium for lymphocytes

Serum-free media especially developed for lymphocyte culture in vitro

Since the end of the last century, the research team of BioEngine has been engaged in the research of in vitro culture of immune cells and has published many academic papers. The team began the independent development of serum-free media for lymphocyte culture in vitro in 2015. HIPP-T006 Lymphocyte serum-free medium is a product free of serum with a limited chemical composition developed by BioEngine according to the characteristics of blood cell growth and differentiation with independent intellectual property rights. HIPP-T006 has a clear composition and uses high-quality standard raw materials to ensure maximum safety and reliability of the production process. It is suitable for in vitro serum-free/reduced culture and amplification of primary cells such as PBMC, T cells, and NK cells as well as cell lines such as NK-92, NK-92MI, K562, and Jurakt.

HIPP-T006 serum-free medium for lymphocytes

Features

Xeno-free, contains no growth factors, synthetic activators, or undefined components

Universality and flexibility: Applicable for the culture of T-cell, NK cell, CAR-T, CIK, CAR-NK, hematopoietic stem cells and other types of cell lines (NK-92, NK-92MI, K562, Jurkat, etc.)

Allowing for direct use in culturing cell lines without the addition of serum, plasma or serum substitutes, to achieve the same culture results as serum-containing media

Better than similar domestic and foreign brands under the same conditions in culturing primary cells

Advantages

Maximum viable cell density up to 5×106 cells/ml under static culture conditions;

Manufactured under the cGMP guidelines and ISO13485 quality system, meet regulatory requirements in different countries;

Use of fully automatic filling lines, to meet the needs of the customer’s dosage and ensure the timeliness of supply and inter-batch stability;

A direct substitute for similar domestic and foreign brands, producing better culture results than their counterparts;

Comprehensive set of DMR documents for audit.

Order Information
Basal Medium
ApplicationProduct NameCat. No.SizeFormProduct Instruction (pdf)Inquiries /Sample application
T-cell, NK cell, CAR-T, CIK, CAR-NK-92, hematopoietic stem cells and other types of cell lines (NK-92, NK-92MI, K562, Jurkat, etc.) HIPP-T006 Lymphocyte Serum-free Medium EXP0105301 1 L
Liquid (non phenol red)
Inquiries /Sample application
EXP0101304 1 L
Liquid (containing phenol red)
Performance
Cell and Gene Therapy

CIK cell culture

HIPP-T006 with 5% fetal bovine serum supported the efficient expansion of CIK cells induced from PBMCs. The cell expansion fold and proportion of CD3+CD56+ cells after 14 days of culture under the same conditions were higher than those of similar imported brands with 5% fetal bovine serum.

 

NK cell culture

HIPP-T006 with 5% fetal bovine serum supported the efficient expansion of NK cells induced from PBMCs. The cell expansion fold and proportion of CD3-CD56+ cells after 14 days of culture under the same conditions were higher than those of similar imported brands with 5% fetal bovine serum.

 

NK-92 cell culture

HIPP-T006 with no serum supports the efficient expansion of NK-92 cells and maintains NK-92 cells phenotype without loss, which is the same as α-MEM supplemented with 12.5% fetal bovine serum and 12.5% horse serum.

Let us contact you

We have a professional technical team to provide high quality one-stop cell culture service from process development
and optimization, medium formulation design to medium processing and manufacturing for all biopharmaceutical companies.

If you have any question, please click "Quick Message" and leave your message,  we will reply to you as soon as possible.
If anything urgent, please call (86)21-68582660-2792.

BioEngine

3F&4F, Building 3, Lane 396, Lvzhou Ring Road, Minhang District, Shanghai, PRC

(86)21-68582660-2792

globalsales@bio-engine.com.cn

Work hours:9:00-17:00