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Vigor series serum-free medium for insect cells

Serum-free media specially developed for efficient large-scale culture of insect cells

BioEngine team started to research techniques for the culture of insect cells (Sf9, High five) in 1995 and successfully developed the first serum-free medium for insect cells in 1998. Based on more than 20 years of technology accumulation and an AI high-throughput data screening platform, BioEngine now releases the latest Vigor series serum-free media for insect cells to meet customers' demand for high-efficient large-scale culture of insect cells. The Vigor series serum-free media for insect cells have been applied in large-scale manufacturing of a variety of products including COVID-19 vaccines and VLPs.

Vigor series serum-free medium for insect cells

Features

Serum-free

Animal-derived component-free

Protein-free

Available for high-density culture of insect cells

Available for high protein expression in insect cells

Available in powder and liquid format

Suitable for both Sf9 and High five cells

Advantages

Animal-derived component-free; TSE/BSE statement available on demand;

Distinctive culture results proven in numerous studies;

Optional powder media for use in large-scale manufacturing with easy preparation procedures;

Powder media capable of a single batch size of 60,000 L;

Excellent inter-batch consistency (CPK*>1.33);

Full traceability by EU-certified ISO13485:2016 Quality Management System;

Complete clinical application supporting documents can be provided. *CPK: Process Capability Index. CPK>1.33 indicates good process control and small inter-batch difference of products.

Order Information
Basal Medium
ProductCat. No.FormSizePackagingProduct Instruction (pdf)NotesInquiries /Sample application
Vigor-S101 Insect Cell Serum-free Medium EXP0118601 Liquid 1 L Bottle

·SF,PF,ADCF

·Suitable for Sf9 cells

·Support the production of subunit or VLP vaccines such as Porcine circovirus,Classical Swine Fever virus,and Porcine parvovirus

·Supports recombinant protein expression

 Inquiries /Sample application
Vigor-S201S Insect Cell Serum-free Medium EXP0107404 Powder 100 L Barrel  

·SF,PF,ADCF

·Suitable for High Five cells

·Support the production of subunit or VLP vaccines such as Porcine circovirus,Classical Swine Fever virus,and Porcine parvovirus

·Supports recombinant protein expression
EXP0107403 Powder 5 L Bag
Vigor-S101S Insect Cell Serum-free Medium EXP0125901 Powder 100 L Bag

·SF,PF,ADCF

·Suitable for Sf9 cells

·Support the production of subunit or VLP vaccines such as Porcine circovirus,Classical Swine Fever virus,and Porcine parvovirus

·Supports recombinant protein expression
EXP0125902 Powder 5 L Bag
Vigor-S101S Additive EXP0107501 Liquid 100 mL Bottle ·The additive is added to Vigor-S101S or Vigor-S201S medium at a ratio of 1:1,000.
EXP0107504 Liquid 50 mL Bottle
*DrivingM® is a registered trademark of Shanghai BioEngine Sci-Tech Co., Ltd.
   The powder culture medium is used in combination with the corresponding 1000 × additives.
Performance
Vaccines

Inoculated with 1×106 cells/ml and passaged every 48 h in Vigor-S101S, Sf9 cell density reached 3-5×106 cells/ml (doubling time of 21-30 h), and High five cell density reached 5-7×106 cells/ml (doubling time of 17-21 h). Indicating Vigor-S101S allows for long-term stable passage.

 

Sf9 cells in Vigor-S101S reached a maximum density of 12-14×106 cells/ml, with a faster growth rate, higher density, and longer maintenance time than in a global brand medium.
The density of High five cells in Vigor-S101S reached a maximum of 9-11×106 cells/ml, which was consistent with the level in a medium of a well-known brand dedicated to High five culture.

 

Vigor-S101S outperformed similar products on the market in terms of titer when using Sf9 cells to produce baculovirus.

Vigor-S101S showed advantages in expressing protein yield using High five cells..

Documents
The brochure of Vigor series serum-free medium for insect cellsView
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3F&4F, Building 3, Lane 396, Lvzhou Ring Road, Minhang District, Shanghai, PRC

(86)21-68582660-2792

globalsales@bio-engine.com.cn

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